A Fluorescent Hydrogel-Based Flow Cytometry High-Throughput Screening Platform for Hydrolytic Enzymes
نویسندگان
چکیده
منابع مشابه
A fluorescent hydrogel-based flow cytometry high-throughput screening platform for hydrolytic enzymes.
Screening throughput is a key in directed evolution experiments and enzyme discovery. Here, we describe a high-throughput screening platform based on a coupled reaction of glucose oxidase and a hydrolase (Yersinia mollaretii phytase [YmPh]). The coupled reaction produces hydroxyl radicals through Fenton's reaction, acting as initiator of poly(ethyleneglycol)-acrylate-based polymerization incorp...
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ژورنال
عنوان ژورنال: Chemistry & Biology
سال: 2014
ISSN: 1074-5521
DOI: 10.1016/j.chembiol.2014.10.018